Rati was born in a Brahmin family on 11 September 1982 in Assam. Her parents originally belong to Patna, Bihar Rati's nickname is "Pammi". Her father and brother own a construction business. Rati is very close to her father and considers him her ideal.
Rati always wanted to go into business. But fate had other plans. Rati came to Mumbai from Delhi and got noticed by Ajay Sinha, but her project with him didn't work out. Rati tried her luck with other production houses.
Rati made her television debut with Zee TV's Shaadi Street and also acted in Har Ghar Kuch Kehta Hai, where she played a character called Prarthna.
Rati Pandey then gained popularity by playing the role of Nupur on Miley Jab Hum Tum. By the end of Season 1 her character was dead but she was brought back on the show, which ended in November.
Rati Pandey participated in the popular dance show Nachle Ve with Saroj Khan.
Rati was highly appreciated for her double role in Zee TV's celebrated show Hitler Didi which made her won many awards.